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Human IL-6R(Interleukin 6 Receptor) ELISA Kit
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ELK11855
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human IL-6R(Interleukin 6 Receptor) ELISA Kit
Reactivity: Human
Alternative Names: IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80;
Assay Type: Sandwich
Sensitivity: 0.45 ng/mL
Standard: 30 ng/mL
Detection Range: 0.47-30 ng/mL
Sample Type: Serum, plasma
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Infection immunity;
Uniprot ID: P08887
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
30.00 2.204 2.111
15.00 1.657 1.564
7.50 1.185 1.092
3.75 0.805 0.712
1.88 0.553 0.460
0.94 0.347 0.254
0.47 0.196 0.103
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6R in samples.
Matrix Recovery range Average
serum(n=5) 96-105% 101%
EDTA plasma(n=5) 85-98% 92%
Heparin plasma(n=5) 85-93% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-95% 85-93% 79-88% 96-103%
EDTA plasma(n=5) 96-105% 81-90% 87-99% 87-99%
Heparin plasma(n=5) 83-93% 91-101% 83-96% 95-105%