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Human NiV-G(Nipah Virus Glycoprotein G) ELISA Kit
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ELK11852
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human NiV-G(Nipah Virus Glycoprotein G) ELISA Kit
Reactivity: Human
Alternative Names: Nipah Virus Glycoprotein G
Assay Type: Sandwich
Sensitivity: 8.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma
Assay Length: 3.5h
Research Area: Microbiology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NiV-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NiV-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NiV-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NiV-G in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.245 2.152
500.00 1.643 1.550
250.00 1.102 1.009
125.00 0.837 0.744
62.50 0.395 0.302
31.25 0.239 0.146
15.63 0.223 0.130
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NiV-G and the recovery rates were calculated by comparing the measured value to the expected amount of NiV-G in samples.
Matrix Recovery range Average
serum(n=5) 85-98% 92%
EDTA plasma(n=5) 95-106% 101%
Heparin plasma(n=5) 83-91% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NiV-G and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 77-86% 88-99% 79-85% 85-92%
EDTA plasma(n=5) 95-107% 77-90% 78-92% 78-92%
Heparin plasma(n=5) 92-104% 85-98% 94-107% 85-98%