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Simian Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
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ELK11694
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: Simian Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
Reactivity: Simian
Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
Standard: 25 ng/mL
Detection Range: 0.39-25 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
25.00 2.379 2.297
12.50 1.536 1.454
6.25 1.243 1.161
3.13 0.872 0.790
1.56 0.541 0.459
0.78 0.385 0.303
0.39 0.215 0.133
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lptn/LTN/XCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lptn/LTN/XCL1 in samples.
Matrix Recovery range Average
serum(n=5) 84-98% 91%
EDTA plasma(n=5) 92-106% 99%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lptn/LTN/XCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-100% 84-98% 97-101%
EDTA plasma(n=5) 83-96% 81-94% 86-98% 92-101%
Heparin plasma(n=5) 82-96% 92-103% 90-99% 90-99%