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Simian TSP-1(Thrombospondin-1) ELISA Kit
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ELK11665
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Simian TSP-1(Thrombospondin-1) ELISA Kit
Reactivity: Simian
Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type: Sandwich
Sensitivity: 0.46 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample Type: Plasma
Assay Length: 3.5h
Research Area: Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.328 2.247
50.00 1.593 1.512
25.00 1.226 1.145
12.50 0.806 0.725
6.25 0.599 0.518
3.13 0.252 0.171
1.56 0.194 0.113
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 78-90% 84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-90% 90-99% 95-106% 87-101%
EDTA plasma(n=5) 83-94% 85-96% 92-103% 86-97%
Heparin plasma(n=5) 93-105% 82-93% 95-104% 92-102%