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Simian TSLP(Thymic Stromal Lymphopoietin) ELISA Kit
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ELK11613
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Simian TSLP(Thymic Stromal Lymphopoietin) ELISA Kit
Reactivity: Simian
Alternative Names: Tslp
Assay Type: Sandwich
Sensitivity: 6.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TSLP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TSLP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TSLP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TSLP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.389 2.302
500.00 1.687 1.600
250.00 1.274 1.187
125.00 0.847 0.760
62.50 0.538 0.451
31.25 0.357 0.270
15.63 0.236 0.149
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TSLP and the recovery rates were calculated by comparing the measured value to the expected amount of TSLP in samples.
Matrix Recovery range Average
serum(n=5) 96-107% 102%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 88-103% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSLP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-94% 89-99% 83-101% 88-96%
EDTA plasma(n=5) 95-104% 93-105% 82-101% 91-103%
Heparin plasma(n=5) 87-99% 85-93% 79-96% 88-98%