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Simian Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
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ELK11552
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Simian Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit
Reactivity: Simian
Alternative Names: Amyloid Beta Peptide 1-40
Assay Type: Sandwich
Sensitivity: 4.48 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.332 2.238
500.00 1.597 1.503
250.00 1.243 1.149
125.00 0.875 0.781
62.50 0.627 0.533
31.25 0.324 0.230
15.63 0.212 0.118
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 93-105% 99%
Heparin plasma(n=5) 83-98% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 89-98% 83-103% 98-105%
EDTA plasma(n=5) 82-93% 96-105% 87-98% 85-97%
Heparin plasma(n=5) 97-106% 82-90% 92-101% 85-92%