Products

Simian I-PTH(IntactPaSimianhormone) ELISA Kit
One-click to copy product information
ELK11548
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual MSDS Send email to us

Overview

Product name: Simian I-PTH(IntactPaSimianhormone) ELISA Kit
Reactivity: Simian
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type: Sandwich
Sensitivity: 4.81 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.328 2.235
500.00 1.597 1.504
250.00 1.283 1.190
125.00 0.892 0.799
62.50 0.521 0.428
31.25 0.301 0.208
15.63 0.226 0.133
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 96-104% 100%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 85-94% 87-101% 86-97%
EDTA plasma(n=5) 84-94% 85-103% 94-105% 92-101%
Heparin plasma(n=5) 87-103% 81-95% 82-90% 85-92%