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Simian CYFRA21-1(CytokeSimianin Fragment Antigen 21-1) ELISA Kit
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ELK11530
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Simian CYFRA21-1(CytokeSimianin Fragment Antigen 21-1) ELISA Kit
Reactivity: Simian
Alternative Names: CYFRA21-1
Assay Type: Sandwich
Sensitivity: 0.117 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CYFRA21-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CYFRA21-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CYFRA21-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.379 2.297
10.00 1.677 1.595
5.00 1.263 1.181
2.50 0.872 0.790
1.25 0.614 0.532
0.63 0.315 0.233
0.31 0.212 0.130
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CYFRA21-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYFRA21-1 in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 83-96% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYFRA21-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-100% 88-99% 81-93% 89-97%
EDTA plasma(n=5) 92-101% 82-98% 89-97% 85-93%
Heparin plasma(n=5) 87-103% 78-91% 83-97% 85-102%