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Simian RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit
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ELK11469
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Simian RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit
Reactivity: Simian
Alternative Names: CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
Assay Type: Sandwich
Sensitivity: 31.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.379 2.306
2500.00 1.436 1.363
1250.00 1.216 1.143
625.00 0.855 0.782
312.50 0.539 0.466
156.25 0.354 0.281
78.13 0.235 0.162
0.00 0.073 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant RANTES and the recovery rates were calculated by comparing the measured value to the expected amount of RANTES in samples.
Matrix Recovery range Average
serum(n=5) 86-98% 92%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 93-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RANTES and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 85-96% 86-95% 90-102%
EDTA plasma(n=5) 89-97% 85-94% 87-96% 85-97%
Heparin plasma(n=5) 85-92% 79-96% 89-101% 85-92%