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Human Anti-CD74(Anti-HLA class II histocompatibility antigen gamma chain Antibody) ELISA Kit
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ELK11340
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human Anti-CD74(Anti-HLA class II histocompatibility antigen gamma chain Antibody) ELISA Kit
Reactivity: Human
Alternative Names: CD74 antibody;DHLAG antibody
Assay Type: Sandwich
Sensitivity: 0.63 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology
Uniprot ID: P04233
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-CD74. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-CD74. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-CD74, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-CD74 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.236 2.153
50.00 1.613 1.530
25.00 1.136 1.053
12.50 0.866 0.783
6.25 0.655 0.572
3.13 0.357 0.274
1.57 0.215 0.132
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-CD74 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-CD74 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-CD74 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 85-96% 87-101% 89-97%
EDTA plasma(n=5) 85-101% 84-98% 85-93% 86-98%
Heparin plasma(n=5) 86-97% 93-102% 87-98% 83-97%