Products

Pig CK-18(Cytokeratin 18) ELISA Kit
One-click to copy product information
ELK11330
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
Send email to us

Overview

Product name: Pig CK-18(Cytokeratin 18) ELISA Kit
Reactivity: Pig
Alternative Names: Cell proliferation inducing gene 46 protein ; Cell proliferation inducing protein 46 ; Cell proliferation-inducing gene 46 protein ; CK 18 ; CK-18 ; CK18 ; CYK 18 ; CYK18 ; Cytokeratin 18 ; Cytokeratin endo B ; Cytokeratin-18 ; K 18 ; K18 ; K1C18_HUMAN ; KA18 ; Keratin 18 ; Keratin 18, type I ; Keratin D ; keratin, type I cytoskeletal 18 ; Keratin-18 ; Krt18
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cancer of the liver; Viral hepatitis
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CK-18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CK-18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CK-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CK-18 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.135 2.053
5.00 1.668 1.586
2.50 1.186 1.104
1.25 0.813 0.731
0.63 0.595 0.513
0.32 0.319 0.237
0.16 0.202 0.120
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CK-18 and the recovery rates were calculated by comparing the measured value to the expected amount of CK-18 in samples.
Matrix Recovery range Average
serum(n=5) 79-93% 86%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 81-98% 81-94% 83-96%
EDTA plasma(n=5) 81-93% 87-101% 96-105% 96-105%
Heparin plasma(n=5) 99-106% 78-92% 87-95% 82-94%