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Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
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ELK11308
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
Reactivity: Human
Alternative Names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1
Assay Type: Sandwich
Sensitivity: 0.121 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.154 2.058
10.00 1.647 1.551
5.00 1.157 1.061
2.50 0.726 0.630
1.25 0.514 0.418
0.63 0.318 0.222
0.32 0.216 0.120
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant p-AMPK and the recovery rates were calculated by comparing the measured value to the expected amount of p-AMPK in samples.
Matrix Recovery range Average
serum(n=5) 93-101% 97%
EDTA plasma(n=5) 85-103% 94%
Heparin plasma(n=5) 95-105% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-AMPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 86-97% 91-102% 81-97% 93-107%
Heparin plasma(n=5) 82-94% 83-96% 91-103% 82-99%