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Human pro interleukin 1 beta (pro-IL1b) ELISA Kit
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ELK11296
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48 T $320.00
96 T $458.00
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Overview

Product name: Human pro interleukin 1 beta (pro-IL1b) ELISA Kit
Reactivity: Human
Alternative Names: pro-IL1B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β
Assay Type: Sandwich
Sensitivity: 11.2 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.204 2.111
1000.00 1.587 1.494
500.00 1.106 1.013
250.00 0.826 0.733
125.00 0.585 0.492
62.50 0.326 0.233
31.25 0.236 0.143
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant pro-IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of pro-IL1b in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 93-101% 97%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pro-IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 87-98% 89-98% 95-103%
EDTA plasma(n=5) 99-106% 78-92% 85-105% 92-104%
Heparin plasma(n=5) 91-103% 87-98% 92-99% 86-93%