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Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
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ELK11289
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human HLA-DRA(HLA class II histocompatibility antigen, DR alpha chain) ELISA Kit
Reactivity: Human
Alternative Names: MHC class II antigen DRA;HLA-DRA;HLA-DRA1
Assay Type: Sandwich
Sensitivity: 0.053 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology
Uniprot ID: P01903
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-DRA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-DRA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-DRA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-DRA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.168 2.110
5.00 1.319 1.261
2.50 0.773 0.715
1.25 0.418 0.360
0.63 0.251 0.193
0.32 0.185 0.127
0.16 0.122 0.064
0.00 0.058 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HLA-DRA and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-DRA in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 84-97% 90%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-DRA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 81-94% 86-98% 92-101%
EDTA plasma(n=5) 85-96% 86-95% 90-102% 81-90%
Heparin plasma(n=5) 87-95% 83-96% 92-102% 82-93%