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Human lambda immunoglobulin light chain(λ-IgLC) ELISA Kit
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ELK11270
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human lambda immunoglobulin light chain(λ-IgLC) ELISA Kit
Reactivity: Human
Alternative Names: lambda immunoglobulin light chain, lambda-IgLC
Assay Type: Competitive Inhibition
Sensitivity: 0.12 µg/mL
Standard: 20 µg/mL
Detection Range: 0.32-20 µg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Immunology
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human λ-IgLC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human λ-IgLC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human λ-IgLC in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
20.00 0.191
10.00 0.357
5.00 0.655
2.50 0.842
1.25 1.127
0.63 1.511
0.32 1.871
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant λ-IgLC and the recovery rates were calculated by comparing the measured value to the expected amount of λ-IgLC in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 78-95% 86%
Heparin plasma(n=5) 87-95% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of λ-IgLC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-96% 87-98% 92-103% 88-97%
EDTA plasma(n=5) 85-93% 98-105% 87-98% 95-102%
Heparin plasma(n=5) 84-99% 81-95% 98-107% 96-101%