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Rat CA15-3(Carbonhydrate Antigen 15-3) Microsample ELISA Kit
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ELK11235MS
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: Rat CA15-3(Carbonhydrate Antigen 15-3) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: CA15-3
Assay Type: Sandwich
Sensitivity: 3.74 pg/mL
Standard: 500 pg/mL
Detection Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
500.00 2.125 2.031
250.00 1.611 1.517
125.00 1.284 1.190
62.50 0.792 0.698
31.25 0.431 0.337
15.63 0.360 0.266
7.81 0.256 0.162
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CA15-3 and the recovery rates were calculated by comparing the measured value to the expected amount of CA15-3 in samples.
Matrix Recovery range Average
serum(n=5) 81-87% 84%
EDTA plasma(n=5) 91-111% 101%
Heparin plasma(n=5) 91-104% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA15-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-114% 81-97% 90-120% 91-107%
EDTA plasma(n=5) 82-94% 82-96% 89-110% 87-109%
Heparin plasma(n=5) 85-120% 83-96% 82-119% 87-118%

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