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Cattle IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
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ELK11156
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48 T $412.00
96 T $588.00
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Overview

Product name: Cattle IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
Reactivity: Cattle
Alternative Names: BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
Assay Type: Sandwich
Sensitivity: 0.129 µg/mLl
Standard: 20 µg/mL
Detection Range: 0.312-20 µg/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;
Uniprot ID: P20959
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IGFBP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IGFBP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IGFBP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IGFBP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
20.00 2.035 1.942
10.00 1.625 1.532
5.00 1.088 0.995
2.50 0.828 0.735
1.25 0.483 0.390
0.63 0.363 0.270
0.32 0.288 0.195
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IGFBP3 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP3 in samples.
Matrix Recovery range Average
serum(n=5) 82-98% 90%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 87-102% 85-96% 87-101%
EDTA plasma(n=5) 88-97% 86-92% 95-103% 98-106%
Heparin plasma(n=5) 85-94% 87-96% 82-95% 81-93%