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Human E-Cad(E-Cadherin) Microsample ELISA Kit
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ELK11056MS
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Human E-Cad(E-Cadherin) Microsample ELISA Kit
Reactivity: Human
Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Uniprot ID: P12830
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.216 2.088
10.00 1.749 1.621
5.00 1.260 1.132
2.50 0.807 0.679
1.25 0.536 0.408
0.63 0.358 0.230
0.31 0.254 0.126
0.00 0.128 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
Matrix Recovery range Average
serum(n=5) 84-109% 97%
EDTA plasma(n=5) 94-116% 105%
Heparin plasma(n=5) 80-90% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 83-120% 95-104% 91-118%
EDTA plasma(n=5) 80-94% 90-111% 93-110% 84-114%
Heparin plasma(n=5) 94-115% 81-116% 87-112% 82-92%

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