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Mouse Lp-a(Lipoprotein a) ELISA Kit
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ELK11050
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse Lp-a(Lipoprotein a) ELISA Kit
Reactivity: Mouse
Alternative Names: LP; AK38; APOA
Assay Type: Sandwich
Sensitivity: 0.128 µg/mL
Standard: 20 µg/mL
Detection Range: 0.312-20 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lp-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lp-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lp-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lp-a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
20.00 2.132 2.037
10.00 1.628 1.533
5.00 1.096 1.001
2.50 0.963 0.868
1.25 0.595 0.500
0.63 0.331 0.236
0.32 0.235 0.140
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lp-a and the recovery rates were calculated by comparing the measured value to the expected amount of Lp-a in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lp-a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 88-95% 96-102% 83-97%
EDTA plasma(n=5) 95-103% 88-96% 81-95% 91-102%
Heparin plasma(n=5) 89-97% 86-102% 94-106% 85-97%