Mouse Cit H3(Citrullinated Histone H3) ELISA Kit
ELK11035 Size： |
 Price： |
|---|---|
| 48 T | $265.00 |
| 96 T | $376.00 |
Manual Overview
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.172 | 2.089 |
| 500.00 | 1.602 | 1.519 |
| 250.00 | 1.235 | 1.152 |
| 125.00 | 0.963 | 0.880 |
| 62.50 | 0.492 | 0.409 |
| 31.25 | 0.347 | 0.264 |
| 15.63 | 0.219 | 0.136 |
| 0.00 | 0.083 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Cit H3 and the recovery rates were calculated by comparing the measured value to the expected amount of Cit H3 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 89-103% | 96% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 90-105% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cit H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 78-92% | 82-96% | 86-99% | 93-101% |
| EDTA plasma(n=5) | 83-96% | 86-95% | 90-102% | 81-93% |
| Heparin plasma(n=5) | 79-91% | 89-103% | 82-95% | 86-99% |