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Horse a2M(Alpha-2-Macroglobulin) ELISA Kit
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ELK10958
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48 T $265.00
96 T $376.00
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Overview

Product name: Horse a2M(Alpha-2-Macroglobulin) ELISA Kit
Reactivity: Horse
Alternative Names: α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Assay Type: Sandwich
Sensitivity: 2.16 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample type: serum, plasma, urine and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse a2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse a2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse a2M in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
500.00 2.056 1.971
250.00 1.613 1.528
125.00 1.118 1.033
62.50 0.836 0.751
31.25 0.509 0.424
15.63 0.302 0.217
7.82 0.186 0.101
0.00 0.085 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant a2M and the recovery rates were calculated by comparing the measured value to the expected amount of a2M in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 81-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of a2M and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 89-103% 97-105% 91-99%
EDTA plasma(n=5) 86-96% 93-104% 89-97% 85-94%
Heparin plasma(n=5) 88-95% 96-102% 89-103% 89-96%