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Mouse Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit
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ELK10951MS
Size： Price：
48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: Mouse Lptn/LTN/XCL1 (Lymphotactin) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Uniprot ID: P47993
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.096 2.002
5.00 1.525 1.431
2.50 1.112 1.018
1.25 0.868 0.774
0.63 0.461 0.367
0.31 0.306 0.212
0.16 0.264 0.170
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lptn/LTN/XCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lptn/LTN/XCL1 in samples.
Matrix Recovery range Average
serum(n=5) 86-107% 97%
EDTA plasma(n=5) 87-118% 103%
Heparin plasma(n=5) 86-118% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lptn/LTN/XCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-113% 80-107% 85-112% 91-104%
EDTA plasma(n=5) 87-95% 90-98% 94-107% 80-100%
Heparin plasma(n=5) 85-109% 84-102% 94-111% 84-96%

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