Products

Mouse p-cresol Microsample ELISA Kit
One-click to copy product information
ELK10942MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual Send email to us

Overview

Product name: Mouse p-cresol Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: 4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol
Assay Type: Sandwich
Sensitivity: 3.8 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.118 2.016
500.00 1.690 1.588
250.00 1.093 0.991
125.00 0.911 0.809
62.50 0.602 0.500
31.25 0.355 0.253
15.63 0.207 0.105
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant p-cresol and the recovery rates were calculated by comparing the measured value to the expected amount of p-cresol in samples.
Matrix Recovery range Average
serum(n=5) 85-100% 93%
EDTA plasma(n=5) 84-92% 88%
Heparin plasma(n=5) 94-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-cresol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-110% 95-116% 95-108% 87-111%
EDTA plasma(n=5) 91-105% 86-103% 87-118% 89-99%
Heparin plasma(n=5) 81-110% 82-101% 94-112% 91-101%

Product Components