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Mouse p-cresol ELISA Kit
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ELK10942
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse p-cresol ELISA Kit
Reactivity: Mouse
Alternative Names: 4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol
Assay Type: Sandwich
Sensitivity: 3.6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.179 2.075
500.00 1.687 1.583
250.00 1.075 0.971
125.00 0.892 0.788
62.50 0.614 0.510
31.25 0.354 0.250
15.63 0.208 0.104
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant p-cresol and the recovery rates were calculated by comparing the measured value to the expected amount of p-cresol in samples.
Matrix Recovery range Average
serum(n=5) 85-95% 90%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-cresol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 85-101% 86-98% 96-107%
EDTA plasma(n=5) 86-93% 88-92% 86-97% 90-101%
Heparin plasma(n=5) 92-102% 81-93% 86-98% 91-103%