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Rat F-Testo(Free Testosterone) ELISA Kit
ELK10934
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48 T $265.00
96 T $376.00
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Overview

Product name: Rat F-Testo(Free Testosterone) ELISA Kit
Reactivity: Rat
Alternative Names: F-TESTO; Free Testosterone
Assay Type: Competitive Inhibition
Sensitivity: 16.3 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat F-Testo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 0.227
1000.00 0.332
500.00 0.621
250.00 0.846
125.00 1.139
62.50 1.565
31.25 1.755
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F-Testo and the recovery rates were calculated by comparing the measured value to the expected amount of F-Testo in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 82-95% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-Testo and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 92-105% 81-93% 93-102%
EDTA plasma(n=5) 89-101% 84-101% 95-104% 88-105%
Heparin plasma(n=5) 86-94% 91-104% 87-98% 95-99%