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Human MCPIP(Monocyte Chemoattractant Protein-induced Protein 1) ELISA Kit
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ELK10915
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48 T $320.00
96 T $458.00
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Overview

Product name: Human MCPIP(Monocyte Chemoattractant Protein-induced Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: Ribonuclease ZC3H12A; MCP-induced protein 1; Zinc finger CCCH domain-containing protein 12A; MCPIP1
Assay Type: Sandwich
Sensitivity: 11.5 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Uniprot ID: Q5D1E8
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCPIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCPIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCPIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCPIP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 2.345 2.259
1000.00 1.763 1.677
500.00 1.138 1.052
250.00 0.861 0.775
125.00 0.438 0.352
62.50 0.304 0.218
31.25 0.189 0.103
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MCPIP and the recovery rates were calculated by comparing the measured value to the expected amount of MCPIP in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MCPIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-99% 83-95% 88-94% 96-101%
EDTA plasma(n=5) 90-99% 83-97% 81-103% 89-101%
Heparin plasma(n=5) 85-94% 90-99% 95-102% 82-90%