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Mouse Ab1-42(Amyloid Beta Peptide 1-42) Microsample ELISA Kit
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ELK10615MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse Ab1-42(Amyloid Beta Peptide 1-42) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Assay Type: Sandwich
Sensitivity: 4.34 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.101 2.011
500.00 1.676 1.586
250.00 1.261 1.171
125.00 0.843 0.753
62.50 0.445 0.355
31.25 0.361 0.271
15.63 0.222 0.132
0.00 0.090 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Ab1-42 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-42 in samples.
Matrix Recovery range Average
serum(n=5) 85-113% 99%
EDTA plasma(n=5) 92-103% 98%
Heparin plasma(n=5) 92-112% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-42 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 94-107% 84-119% 93-112% 85-108%
EDTA plasma(n=5) 82-99% 81-92% 82-92% 92-109%
Heparin plasma(n=5) 95-111% 94-109% 83-107% 87-97%

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