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Rat BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
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ELK10610
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Rat BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Rat
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.11 µg/mL
Standard: 20 µg/mL
Detection Range: 0.32-20 µg/mL
Sample type: serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Uniprot ID: P07151
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
20.00 2.108 2.004
10.00 1.645 1.541
5.00 1.167 1.063
2.50 0.933 0.829
1.25 0.538 0.434
0.63 0.324 0.220
0.32 0.226 0.122
0.00 0.104 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 85-97% 91%
Heparin plasma(n=5) 91-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-92% 96-102% 81-93% 79-91%
EDTA plasma(n=5) 89-101% 85-96% 95-104% 82-90%
Heparin plasma(n=5) 85-93% 87-96% 97-105% 95-102%