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Mouse VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
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ELK10552
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: Mouse VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Reactivity: Mouse
Alternative Names: CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5
Assay Type: Sandwich
Sensitivity: 1.21 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Uniprot ID: P55284
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.153 2.065
100.00 1.628 1.540
50.00 1.052 0.964
25.00 0.863 0.775
12.50 0.527 0.439
6.25 0.236 0.148
3.13 0.161 0.073
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 84-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 85-93% 89-97% 85-94%
EDTA plasma(n=5) 87-96% 92-105% 89-102% 92-101%
Heparin plasma(n=5) 88-102% 87-102% 85-96% 87-101%