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Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
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ELK10410MS
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48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Mouse NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 0.31 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 1.980 1.889
25.00 1.610 1.519
12.50 1.111 1.020
6.25 0.747 0.656
3.13 0.606 0.515
1.56 0.238 0.147
0.78 0.170 0.079
0.00 0.091 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.
Matrix Recovery range Average
serum(n=5) 84-104% 94%
EDTA plasma(n=5) 82-98% 90%
Heparin plasma(n=5) 92-119% 106%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-94% 83-98% 85-119% 92-98%
EDTA plasma(n=5) 84-90% 95-115% 81-88% 85-111%
Heparin plasma(n=5) 88-109% 89-110% 90-102% 90-119%

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