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Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
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ELK10409MS
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48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Human NT-ProANP(N-Terminal Pro-Atrial Natriuretic Peptide) Microsample ELISA Kit
Reactivity: Human
Alternative Names: NT-Pro-ANP; N-ANP
Assay Type: Sandwich
Sensitivity: 0.32 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.099 2.010
25.00 1.586 1.497
12.50 1.094 1.005
6.25 0.857 0.768
3.13 0.528 0.439
1.56 0.396 0.307
0.78 0.210 0.121
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProANP in samples.
Matrix Recovery range Average
serum(n=5) 89-96% 93%
EDTA plasma(n=5) 83-130% 107%
Heparin plasma(n=5) 92-113% 103%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-116% 84-116% 84-93% 81-116%
EDTA plasma(n=5) 81-87% 95-103% 84-107% 93-112%
Heparin plasma(n=5) 81-100% 89-100% 90-112% 86-115%

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