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Mouse Cys-C(Cystatin C) ELISA Kit
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ELK10181
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48 T $265.00
96 T $376.00
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Overview

Product name: Mouse Cys-C(Cystatin C) ELISA Kit
Reactivity: Mouse
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage
Assay Type: Sandwich
Sensitivity: 0.49 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Kidney biomarker;Urology;
Uniprot ID: P21460
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.082 1.895
50.00 1.761 1.574
25.00 1.251 1.064
12.50 0.862 0.675
6.25 0.503 0.316
3.13 0.401 0.214
1.57 0.224 0.037
0.00 0.187 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 78-90% 84%
EDTA plasma(n=5) 89-107% 98%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 79-96% 87-103% 90-99%
EDTA plasma(n=5) 88-97% 96-105% 91-103% 89-97%
Heparin plasma(n=5) 85-94% 87-96% 79-95% 95-102%