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Mouse Flt3L(FMS Like Tyrosine Kinase 3 Ligand) Microsample ELISA Kit
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ELK10179MS
Size： Price：
48 T $265.00
96 T $376.00
96 T*5 $1598.00
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Overview

Product name: Mouse Flt3L(FMS Like Tyrosine Kinase 3 Ligand) Microsample ELISA Kit
Reactivity: Mouse
Alternative Names: FLT3LG; FLT3-LG; Flt3-L; FL; Fms-Related Tyrosine Kinase 3 ligand; SL cytokine
Assay Type: Sandwich
Sensitivity: 12.4 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;Immune molecule;Hematology;
Uniprot ID: P49772
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Flt3L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Flt3L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Flt3L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Flt3L in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
2000.00 1.871 1.784
1000.00 1.549 1.462
500.00 1.023 0.936
250.00 0.685 0.598
125.00 0.427 0.340
62.50 0.307 0.220
31.25 0.210 0.123
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Flt3L and the recovery rates were calculated by comparing the measured value to the expected amount of Flt3L in samples.
Matrix Recovery range Average
serum(n=5) 85-90% 88%
EDTA plasma(n=5) 82-107% 95%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Flt3L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 84-109% 89-100% 94-111% 80-87%
EDTA plasma(n=5) 85-111% 95-102% 83-105% 87-104%
Heparin plasma(n=5) 85-115% 85-112% 86-95% 85-109%

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