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Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit
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ELK10154
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48 T $320.00
96 T $458.00
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Overview

Product name: Mouse KL-6(Krebs Von den Lungen-6) ELISA Kit
Reactivity: Mouse
Alternative Names: KL-6; Mucin-1; MUC1
Assay Type: Sandwich
Sensitivity: 8.86 U/mL
Standard: 1500 U/mL
Detection Range: 23.44-1500 U/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Interstitial lung disease
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KL-6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KL-6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
1500.00 2.165 2.070
750.00 1.694 1.599
375.00 1.219 1.124
187.50 0.864 0.769
93.75 0.567 0.472
46.88 0.318 0.223
23.44 0.196 0.101
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 96-107% 102%
Heparin plasma(n=5) 95-104% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 91-101% 87-98% 89-103% 81-96%
EDTA plasma(n=5) 98-107% 93-102% 80-93% 91-100%
Heparin plasma(n=5) 92-105% 85-101% 85-94% 87-96%