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Chicken 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
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ELK10145
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Chicken 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity: Chicken
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type: Competitive Inhibition
Sensitivity: 0.56 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 0.227
50.00 0.386
25.00 0.621
12.50 0.861
6.25 1.163
3.13 1.585
1.57 1.889
0.00 2.325

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
Matrix Recovery range Average
serum(n=5) 92-102% 97%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 85-93% 89-97% 95-102%
EDTA plasma(n=5) 88-104% 87-96% 85-93% 82-90%
Heparin plasma(n=5) 79-96% 89-100% 91-102% 89-97%