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Dog Cys-C(Cystatin C) ELISA Kit
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ELK10127
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48 T $320.00
96 T $458.00
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Overview

Product name: Dog Cys-C(Cystatin C) ELISA Kit
Reactivity: Dog
Alternative Names: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
Assay Type: Sandwich
Sensitivity: 0.64 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Tags & Cell Markers；Cell Biology；Stem Cells；Cardiovascular
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 2.132 2.045
50.00 1.624 1.537
25.00 1.223 1.136
12.50 0.933 0.846
6.25 0.593 0.506
3.13 0.357 0.270
1.57 0.189 0.102
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 94-107% 100%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-103% 87-98% 95-101%
EDTA plasma(n=5) 93-99% 87-101% 85-95% 85-96%
Heparin plasma(n=5) 88-100% 95-103% 89-97% 85-94%