Products

Rat IgG1(Immunoglobulin G1) ELISA Kit
One-click to copy product information
ELK10114
Size： Price：
48 T $320.00
96 T $458.00
Manual MSDS Send email to us

Overview

Product name: Rat IgG1(Immunoglobulin G1) ELISA Kit
Reactivity: Rat
Alternative Names: IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
Assay Type: Sandwich
Sensitivity: 2.41 µg/mL
Standard: 400 µg/mL
Detection Range: 6.25-400 µg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (µg/mL) OD Corrected OD
400.00 2.165 2.086
200.00 1.436 1.357
100.00 1.088 1.009
50.00 0.828 0.749
25.00 0.424 0.345
12.50 0.389 0.310
6.25 0.225 0.146
0.00 0.079 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IgG1 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG1 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 78-93% 85%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-91% 86-92% 89-96% 91-97%
EDTA plasma(n=5) 88-102% 87-102% 85-96% 87-101%
Heparin plasma(n=5) 86-93% 93-101% 86-97% 90-101%