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Human CK 18-M65 (Cytokeratin 18-M65) ELISA Kit
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ELK10045
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48 T $320.00
96 T $458.00
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Overview

Product name: Human CK 18-M65 (Cytokeratin 18-M65) ELISA Kit
Reactivity: Human
Alternative Names: K18-M65; CK-18-M65; CYK18-M65
Assay Type: Sandwich
Sensitivity: 29.8 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Cancer of the liver; Viral hepatitis
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 2.179 2.097
2500.00 1.576 1.494
1250.00 1.224 1.142
625.00 0.876 0.794
312.50 0.585 0.503
156.25 0.324 0.242
78.13 0.258 0.176
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CK 18-M65 and the recovery rates were calculated by comparing the measured value to the expected amount of CK 18-M65 in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK 18-M65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 86-93% 81-94% 83-96%
EDTA plasma(n=5) 83-96% 97-105% 85-99% 79-93%
Heparin plasma(n=5) 90-99% 83-97% 81-103% 78-92%