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Human Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
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ELK10035
Size： Price：
48 T $412.00
96 T $588.00
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Overview

Product name: Human Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit
Reactivity: Human
Alternative Names: Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
Standard: 25 ng/mL
Detection Range: 0.39-25 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;Dermatology;
Uniprot ID: P47992
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
25.00 2.096 2.003
12.50 1.587 1.494
6.25 1.134 1.041
3.13 0.826 0.733
1.57 0.545 0.452
0.79 0.324 0.231
0.40 0.174 0.081
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Lptn/LTN/XCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lptn/LTN/XCL1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lptn/LTN/XCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 87-102% 99-106% 78-92%
EDTA plasma(n=5) 88-95% 92-101% 96-105% 85-92%
Heparin plasma(n=5) 82-96% 87-98% 89-98% 95-103%