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TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit（Activation Required）
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ELK10034
Size： Price：
48 T $320.00
96 T $458.00
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Overview

Product name: TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit（Activation Required）
Reactivity: General
Alternative Names: Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Activated serum, plasma
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF-b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF-b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.258 2.170
5.00 1.593 1.505
2.50 1.136 1.048
1.25 0.847 0.759
0.63 0.518 0.430
0.32 0.385 0.297
0.16 0.226 0.138
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant TGF-b1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGF-b1 in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 87-101% 94%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGF-b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 93-102% 95-103% 93-102%
EDTA plasma(n=5) 82-96% 92-103% 90-99% 89-102%
Heparin plasma(n=5) 85-96% 82-96% 85-98% 95-104%