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Zebrafish NE(Nor-Epinephrine) ELISA Kit
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ELK0956
Size： Price：
48 T $265.00
96 T $376.00
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Overview

Product name: Zebrafish NE(Nor-Epinephrine) ELISA Kit
Reactivity: Zebrafish
Alternative Names: Noradrenaline
Assay Type: Competitive Inhibition
Sensitivity: 25.6 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Infection immunity;Neuro science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish NE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish NE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
5000.00 0.199
2500.00 0.397
1250.00 0.611
625.00 0.823
312.50 1.253
156.25 1.596
78.13 1.863
0.00 2.115

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 80-96% 88%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-95% 89-102% 85-92% 96-104%
EDTA plasma(n=5) 78-92% 83-96% 87-101% 97-106%
Heparin plasma(n=5) 86-97% 95-101% 88-102% 81-93%