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EasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
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ELK087ES
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48 T $412.00
96 T $588.00
96 T*5 $2499.00
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Overview

Product name: EasyStep Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Reactivity: Human
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 37.66 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
6000.00 2.189 2.088
3000.00 1.582 1.481
1500.00 1.243 1.142
750.00 0.935 0.834
375.00 0.503 0.402
187.50 0.285 0.184
93.75 0.219 0.118
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-2Ra/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2Ra/CD25 in samples.
Matrix Recovery range Average
serum(n=5) 81-97% 89%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2Ra/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-96% 85-99% 79-93% 89-97%
EDTA plasma(n=5) 88-105% 86-92% 81-93% 79-97%
Heparin plasma(n=5) 85-102% 87-96% 92-101% 89-104%

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