Products

Human MR-proANP(midregional pro-atrial natriuretic peptide) Microsample ELISA Kit
One-click to copy product information
ELK0872MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
Manual Send email to us

Overview

Product name: Human MR-proANP(midregional pro-atrial natriuretic peptide) Microsample ELISA Kit
Reactivity: Human
Alternative Names: MR-proANP
Assay Type: Sandwich
Sensitivity: 0.05 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-proANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-proANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-proANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-proANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.151 2.054
5.00 1.742 1.645
2.50 1.209 1.112
1.25 0.888 0.791
0.63 0.532 0.435
0.31 0.342 0.245
0.16 0.209 0.112
0.00 0.097 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MR-proANP and the recovery rates were calculated by comparing the measured value to the expected amount of MR-proANP in samples.
Matrix Recovery range Average
serum(n=5) 82-118% 100%
EDTA plasma(n=5) 91-118% 105%
Heparin plasma(n=5) 83-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MR-proANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-107% 91-117% 80-95% 94-117%
EDTA plasma(n=5) 90-95% 94-114% 93-102% 94-103%
Heparin plasma(n=5) 91-115% 87-94% 93-116% 86-108%

Product Components