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Human MR-proANP(midregional pro-atrial natriuretic peptide) ELISA Kit
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ELK0872
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48 T $320.00
96 T $458.00
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Overview

Product name: Human MR-proANP(midregional pro-atrial natriuretic peptide) ELISA Kit
Reactivity: Human
Alternative Names: MR-proANP
Assay Type: Sandwich
Sensitivity: 0.051 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-proANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-proANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-proANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-proANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.126 2.031
5.00 1.741 1.646
2.50 1.185 1.090
1.25 0.864 0.769
0.63 0.539 0.444
0.32 0.347 0.252
0.16 0.213 0.118
0.00 0.095 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MR-proANP and the recovery rates were calculated by comparing the measured value to the expected amount of MR-proANP in samples.
Matrix Recovery range Average
serum(n=5) 85-99% 92%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MR-proANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 86-95% 90-103% 87-90%
EDTA plasma(n=5) 87-94% 85-96% 92-102% 81-90%
Heparin plasma(n=5) 95-103% 89-101% 87-98% 85-92%