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Human Anti-FOLR(folate receptor autoantibodies IgG) ELISA Kit
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ELK0865
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48 T $265.00
96 T $376.00
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Overview

Product name: Human Anti-FOLR(folate receptor autoantibodies IgG) ELISA Kit
Reactivity: Human
Alternative Names: ANTI-FOLR1
Assay Type: Sandwich
Sensitivity: 3.3 ng/mL
Standard: 500 ng/mL
Detection Range: 7.82-500 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-FOLR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-FOLR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-FOLR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-FOLR in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.045 1.962
250.00 1.502 1.419
125.00 1.142 1.059
62.50 0.671 0.588
31.25 0.485 0.402
15.63 0.313 0.230
7.82 0.213 0.130
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-FOLR and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-FOLR in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 83-99% 95%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-FOLR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-93% 96-103% 97-105% 89-97%
EDTA plasma(n=5) 84-102% 87-103% 97-104% 82-97%
Heparin plasma(n=5) 83-92% 91-96% 85-93% 95-101%