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Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit
ELK0858
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48 T $320.00
96 T $458.00
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Overview

Product name: Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit
Reactivity: Human
Alternative Names: IA2 Ab
Assay Type: Sandwich
Sensitivity: 26.8 U/mL
Standard: 4000 U/mL
Detection Range: 62.5-4000 U/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
4000.00 2.054 1.971
2000.00 1.527 1.444
1000.00 1.106 1.023
500.00 0.835 0.752
250.00 0.473 0.390
125.00 0.327 0.244
62.50 0.175 0.092
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IA-2 Ab and the recovery rates were calculated by comparing the measured value to the expected amount of IA-2 Ab in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 91-99% 95%
Heparin plasma(n=5) 80-92% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IA-2 Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-100% 95-103% 89-97% 85-94%
EDTA plasma(n=5) 87-103% 97-105% 79-90% 87-96%
Heparin plasma(n=5) 85-95% 85-96% 93-99% 87-101%