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Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
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ELK0857
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48 T $320.00
96 T $458.00
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Overview

Product name: Human MRPS12(28S ribosomal protein S12, mitochondrial) ELISA Kit
Reactivity: Human
Alternative Names: RPS12; MRPS12; MRPS34; PSMD7; HTR1B
Assay Type: Competitive Inhibition
Sensitivity: 0.28 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids
Assay length: 2h
Research Area: Developmental science;
Uniprot ID: O15235
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 0.199
25.00 0.396
12.50 0.557
6.25 0.833
3.13 1.119
1.57 1.511
0.79 1.755
0.00 2.123

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MRPS12 and the recovery rates were calculated by comparing the measured value to the expected amount of MRPS12 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 78-96% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MRPS12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 87-96% 88-101% 82-95%
EDTA plasma(n=5) 79-92% 88-96% 77-91% 85-93%
Heparin plasma(n=5) 86-97% 93-102% 87-98% 83-97%