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Rat hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
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ELK0844MS
Size： Price：
48 T $320.00
96 T $458.00
96 T*5 $1947.00
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Overview

Product name: Rat hs-cTnT(High Sensitivity Cardiac Troponin T) Microsample ELISA Kit
Reactivity: Rat
Alternative Names: High Sensitivity Cardiac Troponin T
Assay Type: Sandwich
Sensitivity: 5.3 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
1000.00 2.096 2.014
500.00 1.670 1.588
250.00 1.114 1.032
125.00 0.830 0.748
62.50 0.524 0.442
31.25 0.322 0.240
15.63 0.259 0.177
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.
Matrix Recovery range Average
serum(n=5) 92-111% 102%
EDTA plasma(n=5) 82-91% 87%
Heparin plasma(n=5) 92-118% 105%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-105% 87-98% 91-117% 83-120%
EDTA plasma(n=5) 87-111% 90-107% 89-108% 93-108%
Heparin plasma(n=5) 93-108% 81-91% 86-106% 82-91%

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