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Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit
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ELK0814
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48 T $320.00
96 T $458.00
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Overview

Product name: Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit
Reactivity: Human
Alternative Names: ANTI-LKM1
Assay Type: Sandwich
Sensitivity: 1.66 U/mL
Standard: 300 U/mL
Detection Range: 4.69-300 U/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (U/mL) OD Corrected OD
300.00 2.096 2.008
150.00 1.638 1.550
75.00 1.182 1.094
37.50 0.871 0.783
18.75 0.539 0.451
9.38 0.326 0.238
4.69 0.226 0.138
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-LKM1 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-LKM1 in samples.
Matrix Recovery range Average
serum(n=5) 83-98% 90%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-LKM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-105% 87-98% 87-103%
EDTA plasma(n=5) 86-92% 89-103% 89-98% 79-85%
Heparin plasma(n=5) 93-99% 86-101% 87-96% 92-105%