Products

PROA(Protein A) ELISA Kit
One-click to copy product information
ELK0772
Size： Price：
48 T $265.00
96 T $376.00
Manual MSDS Send email to us

Overview

Product name: PROA(Protein A) ELISA Kit
Reactivity: General
Alternative Names: Staphylococcus aureus protein A (SpA)
Assay Type: Competitive Inhibition
Sensitivity: 19.1 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample type: serum, plasma, other biological fluids
Assay length: 2.5h
Research Area: Tumor immunity;Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PROA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PROA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PROA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (pg/mL) OD Corrected OD
3000.00 0.197
1500.00 0.357
750.00 0.611
375.00 0.899
187.50 1.157
93.75 1.585
46.88 1.761
0.00 2.375

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PROA and the recovery rates were calculated by comparing the measured value to the expected amount of PROA in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PROA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 96-105% 92-103% 82-96%
EDTA plasma(n=5) 86-97% 83-96% 91-103% 96-105%
Heparin plasma(n=5) 88-103% 86-97% 87-102% 89-101%